Coding

Part:BBa_K1114408:Design

Designed by: Devina Desai   Group: iGEM13_BostonU   (2013-09-07)


This is a MoClo level 0 destination vector.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 251
    Illegal XbaI site found at 278
    Illegal SpeI site found at 1
    Illegal SpeI site found at 7
    Illegal SpeI site found at 535
    Illegal PstI site found at 290
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 251
    Illegal SpeI site found at 1
    Illegal SpeI site found at 7
    Illegal SpeI site found at 535
    Illegal PstI site found at 290
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 251
    Illegal BamHI site found at 272
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 251
    Illegal XbaI site found at 278
    Illegal SpeI site found at 1
    Illegal SpeI site found at 7
    Illegal SpeI site found at 535
    Illegal PstI site found at 290
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 251
    Illegal XbaI site found at 278
    Illegal SpeI site found at 1
    Illegal SpeI site found at 7
    Illegal SpeI site found at 535
    Illegal PstI site found at 290
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 14
    Illegal BsaI.rc site found at 528


Design Notes

This is a MoClo destination vector containing the lacZ alpha fragment for blue-white screening with fusion sites D on the 5' side and site F on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.

Summary of modifications from original part:

Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites.



Source

iGEM kit

References